For Science

Fellow Scientists:

We had formally proposed splicingcode model in 2007 and published it in 2012. Recently, we have used this model to analyze fusion transcripts and have identified over 1.1 million fusion transcripts. These large numbers of fusion transcripts have indirectly validated the splicingcode’s mathematics model. Understanding this model may help you to make new discoveries and/or to develop new drugs. Following is from "Deciphering Splicing Codes of Spliceosomal Introns":


Fig. Schematic model of a nuclear pre-mRNA splicing pathway involving a splicer RNA (a) and proteins (b). The black and gray boxes represent the 5’ and 3’ exon sequences, respectively, and the shadowed oval represents a core spliceosome. The circled A is the branchpoint adenosine, and gu and ag represent the nucleotides typically present at the 5’ and 3’ ends of introns, respectively. a). Schematic model of E5 and I3 sequences that are recognized by splicer RNAs. The lines represent the intron and putative splicer RNA sequences, respectively. The vertical lines represent base-pairing between the putative splicer RNA and pre-mRNA (although these two cis-elements in a splicer RNA are not expected to be identical). The last nucleotide of the 5’ exon and the last two nucleotides of the intron may lack perfect complementarities. For simplicity, a single splicer RNA has been shown, although the model is compatible with two RNAs (recognizing the 5’ exon and 3’ intron, respectively) in conjunction with other spliceosomal components. This model is conceptually similar to that first proposed by Holliday and Murray. b. Schematic model of E5 and I3 sequences that are recognized by as yet uncharacterized proteins. The E5 interacts in a sequence-specific manner with an as yet uncharacterized protein [1] and I3 is recognized by a different unknown protein (oval). These two proteins interact with each other to assist in bringing together the 5’ and 3’ splice sites.